Journal: iScience

Article Title: Sevoflurane-induced gut microbiota dysbiosis drives adolescent neurobehavioral deficits in neonatal rats: Protective role of eicosapentaenoic acid

doi: 10.1016/j.isci.2025.113657

Figure Lengend Snippet: The gut microbiota metabolites on day 7 after sevoflurane exposure (A–M) Isophthalic acid, N-methyl-DL-alanine, 2-hydroxybutyric acid, (S)- 2-hydroxybutyric acid, lactic acid, phosphate, L-phenylalanine, glyceryl palmitate, eicosapentaenoic acid, L-leucine, tyramine, tetradecanoic acid, and (9Z, 12Z, 15Z)-octadecatrienonic acid. Data are represented as mean ± SEM. n = 7 for Ctrl group, n = 6 for Sev group. ∗ p < 0.05, ∗∗ p < 0.01, Sev vs. Ctrl group.

Article Snippet: Eicosapentaenoic acid (EPA) , TargetMol, Boston, MA, USA , Cat#: T72152.

Techniques:

Journal: iScience

Article Title: Sevoflurane-induced gut microbiota dysbiosis drives adolescent neurobehavioral deficits in neonatal rats: Protective role of eicosapentaenoic acid

doi: 10.1016/j.isci.2025.113657

Figure Lengend Snippet: Effects of eicosapentaenoic acid on neurobehavioral tests in rats exposed to sevoflurane (A) Percentage of open arm entry times of rats in the different groups in the EPM test. (B) Percentage of open arm duration of rats in the different groups in the EPM test. (C) The total distance on the open arm of rats in the different groups in the EPM test. (D) The time of rats in the different groups in the area of stranger 1 and empty cage in step 1 of the TCST. (E) The time of rats in the different groups in the area of familiar and stranger 2 in step 2 of the TCST. (F) Swimming speed of rats in the different groups in the MWM test. (G) Swimming distance of rats in the different groups in the MWM test. (H) The escape latency to find the platform of rats in the different groups in the MWM test. (I) The number of target crossing of rats in the different groups in the MWM test. Data are represented as mean ± SEM. n = 12 per group. ∗ p < 0.05, ∗∗ p < 0.01, Sev+EPA vs. Sev group; ## p < 0.01, Time in area of the stranger 1 vs. Time in area of the empty cage; ▲▲ p < 0.01, Time in area of familiar vs. Time in area of stranger 2.

Article Snippet: Eicosapentaenoic acid (EPA) , TargetMol, Boston, MA, USA , Cat#: T72152.

Techniques:

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 expression was related to poor prognosis in HCC. ( A) Representative images of IDO1 immunofluorescence staining of HCC tissue and paracancerous tissue in patients with HCC and quantitative results of the fluorescence intensity of IDO1. ( B ) Kaplan–Meier curves for OS in HCC patients based on IDO1expression level. ( C ) The IDO1 expression grouped by age, gender ( D ), tumor size ( E ), stage ( F ), AFP ( G ), HBV ( H ), cirrhosis ( I ), metastasis ( J ), relapse ( K ) and HBcAb ( L ). The data were presented as mean ± SEM (* P < 0.05).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Expressing, Immunofluorescence, Staining, Fluorescence

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: The infiltration and prognostic significance of mature DCs subsets in HCC. ( A ) Representative images of immunofluorescence staining of CK, CD11C, MHCII, CD40, CD80, in cancer and paracancerous tissues from HCC patients. ( B ) Quantitative analysis of the percentage of CD11C + , CD11C + MHCII + , CD11C + CD80 + and CD11C + CD40 + DCs. ( C ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + DCs. ( D ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + MHCII + DCs. ( E ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + CD80 + DCs. ( F ) Kaplan–Meier curves for OS in HCC patients based on the percentage of CD11C + CD40 + DCs. ( G ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + MHCII + DCs, according to Spearman correlation analysis. ( H ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + CD80 + DCs, according to Spearman correlation analysis. ( I ) The correlation between the percentage of IDO1 + staining area levels and the percentage of CD11C + CD40 + DCs, according to Spearman correlation analysis. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Immunofluorescence, Staining

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could attenuate the EMT and malignant proliferation of SK-HEP1 in vitro. ( A ) Western blot analysis was used to detect the expression levels of Vimentin and IDO1 in control and experimental SK-HEP1 cells at 24 h after EPA intervention. GAPDH served as a loading control. The density of protein was measured using Quantity One software. ( B ) Western blot analysis was used to detect the expression levels of E-cadherin and PCNA in control and experimental SK-HEP1 cells at 24 h after EPA intervention. α-tubulin served as a loading control. The density of protein was measured using Quantity One software. ( C ) Relative mRNA levels of IDO1, E-cadherin, Vimentin, and PCNA were measured in SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vitro, Western Blot, Expressing, Control, Software

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could improve the malignant biological behavior of SK-HEP1 cells in vitro. ( A ) The proliferation of SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h was evaluated using the Key Fluor 488 Click-iT Edu assay. ( B ) The proliferation rate of SK-HEP1cells in ( A ) was quantified in a bar graph. ( C ) The cell viability of SK-HEP1 cells treated with 0, 2.5, 5, 10, and 20 μM EPA for 24 h was assessed using the CCK-8 assay. The cell viability reported as fold change of EPA-treated vs control cells. ( D ) Representative images from the transwell assay (left) and migrating cells number analysis (right). ( E ) Representative images from the wound healing assay. The data were presented as mean ± SEM (* P < 0.05, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vitro, EdU Assay, CCK-8 Assay, Control, Transwell Assay, Wound Healing Assay

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could delay the subcutaneous tumor formation of SK-HEP-1 cells in vivo. ( A ) Schematic protocol for animal experiment. ( B ) The tumor growth curve of mice and the representative diagrams of tumors in each group. ( C ) Tumor weight of the mice in control and EPA-treated group. ( D ) Immunofluorescence staining of IDO1 in tumor tissues in control and EPA-treated group. ( E ) The serum levels of Trp and Kyn were determined in mice from both the control and EPA-treated groups. ( F ) HE staining of tumor tissues in control and EPA-treated group. ( G ) Immunohistochemistry staining of PCNA in control and EPA-treated group. The data were presented as mean ± SEM (* P < 0.05, ** P <0.01, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vivo, Control, Immunofluorescence, Staining, Immunohistochemistry

Journal: Journal of Hepatocellular Carcinoma

Article Title: Low Indoleamine 2,3-Dioxygenase 1 Expression Enhances Dendritic Cells Response to Tumor Cells Against Hepatocellular Carcinoma

doi: 10.2147/JHC.S530997

Figure Lengend Snippet: IDO1 inhibition could enhance immune cells response to tumor cells in vivo. ( A ) Immunofluorescence staining of CD11C, MHCII,CD80 and CD40 in tumor tissues in control and EPA-treated group. ( B ) Immunofluorescence staining of CD4 and CD8 in tumor tissues in control and EPA-treated group. The data were presented as mean ± SEM (* P < 0.05, *** P < 0.001).

Article Snippet: IDO1 catalytic inhibitor epacadostat (EPA) was purchased from MedChemExpress LLC (Shanghai, China).

Techniques: Inhibition, In Vivo, Immunofluorescence, Staining, Control